FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2239_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2239_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1544715
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT168621.0915929475663797No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC123720.800924442372865No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA110630.716183891526916No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATATT108620.7031717824970949No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT107810.6979280967686596No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG88820.574992798024231No Hit
TTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATA79880.5171180444289076No Hit
TTTCTTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACTGGAG73000.4725790841676296No Hit
GGATATTCGAGAAATTCTTCCCTAGCAGTTCATATCGGAGGCCAGTTGGA68500.4434474967874333No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATATTCGAGA64410.41697012070187706No Hit
GGATACACCATGGACACAGTCAACAGAACACAAAGGGGAATTCTTGAGGA61080.3954127460405317No Hit
TTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATG56910.3684174750682165No Hit
GTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGGCCATGGTG53470.3461479949375775No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA52970.34291115189533344No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG52030.33682588697591465No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG47930.3102837740295135No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC47650.3084711419258569No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG44800.29002113658506584No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA44000.28484218771747544No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG43660.28264113444874944No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT37160.24056217489957696No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA36810.23829638477000614No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG36330.23518901544945184No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG35130.22742059214806615No Hit
CTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCT33710.21822795790809307No Hit
GAACAGGAACAGGATATTCGAGAAATTCTTCCCTAGCAGTTCATATCGGA33470.21667427324781594No Hit
ATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGG33330.21576795719598763No Hit
GTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGA33030.21382585137064117No Hit
AAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCATCAT32990.21356690392726166No Hit
CCATATAGCCATGGAACAGGAACAGGATATTCGAGAAATTCTTCCCTAGC32290.20903532366812No Hit
CGAATAGAGTGCAGCACTTCTGGTACATTTGTTCATCCTCAAGAATTCCC32050.2074816390078429No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA30960.20042532117575088No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG29870.19336900334365886No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT29380.1901968971622597No Hit
CTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGT29270.189484791692966No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC28850.18676584353748105No Hit
TTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGTC28720.1859242643464976No Hit
GTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCC28490.18443531654706533No Hit
TGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTT28240.1828168950259433No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTGCCAGCGCAAAAT28050.18158689466989056No Hit
TCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGGCCATGGTGTC26980.17466005055948833No Hit
GTCTAGGGCCCGAATTGATGCACGGATTGACTTCGAGTCTGGAAGGATTA26770.17330057648174582No Hit
CCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCAG26760.17323583962090094No Hit
ATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAA26650.17252373415160724No Hit
GTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGG26640.17245899729076236No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG25990.16825110133584514No Hit
GATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGA25580.165596890041205No Hit
CCCCCATATAGCCATGGAACAGGAACAGGATATTCGAGAAATTCTTCCCT25400.16443162654599716No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA25370.16423741596346253No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC24760.1602884674519248No Hit
ATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAA24260.15705162440968076No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA24260.15705162440968076No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA23850.15439741311504063No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA23810.15413846567166112No Hit
ATATTATTCACTTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC23580.15264951787222886No Hit
ATGTAGGACCATTCTCTTCCTCCCAACAGCAGGTCACAAGAAGGGTTGCC23340.15109583321195172No Hit
CCCATATAGCCATGGAACAGGAACAGGATATTCGAGAAATTCTTCCCTAG23210.15025425402096826No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT22510.14572267376182663No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG22340.14462214712746363No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC22100.1430684624671865No Hit
TCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCA21890.141708988389444No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC21760.14086740919846055No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTG21110.1366595132435433No Hit
CCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTGCTAGGGAAG21110.1366595132435433No Hit
CTTCTAAGTGTGTGCATATTGCAGCGAACTTATTTGTTTCAATTTTCGGG21000.1359474077742496No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA20920.13542951288749056No Hit
ATGGAGGCCATGGTGTCTAGGGCCCGAATTGATGCACGGATTGACTTCGA20810.13471740741819688No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA20480.13258109101031582No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT20440.13232214356693628No Hit
CTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCT20420.13219266984524652No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG20330.1316100380976426No Hit
GGAACAGGATATTCGAGAAATTCTTCCCTAGCAGTTCATATCGGAGGCCA19640.12714319469934585No Hit
ACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCAATTGAG19610.1269489841168112No Hit
CTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATA19350.1252658257348443No Hit
GGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTGCTAG19330.12513635201315454No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT18720.1211874035016168No Hit
ATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTG18650.12073424547570265No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA18590.12034582431063336No Hit
CTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCA18520.1198926662847192No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT18520.1198926662847192No Hit
GGTTTAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTA18470.11956898198049479No Hit
ATTATATACAAATGTTGCATCTGCAAGATCCATTGGACATGGCCCAGAAC18190.11775634987683811No Hit
GAAAAGACAATGAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAA17860.11562003346895705No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG17800.11523161230388777No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA17520.11341898020023113No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA17350.11231845356586814No Hit
ATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCT17100.11070003204474613No Hit
GAACAGGATATTCGAGAAATTCTTCCCTAGCAGTTCATATCGGAGGCCAG17090.11063529518390125No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG17000.11005266343629731No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC16990.10998792657545244No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA16980.10992318971460756No Hit
CTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCAT16840.10901687366277922No Hit
GATATGAACTGCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGG16510.10688055725489815No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT16380.1060389780639147No Hit
ATGCAATAAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCAT16370.10597424120306982No Hit
AGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGAT16350.10584476748138007No Hit
TCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTA16290.10545634631631078No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC16160.10461476712532733No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT16150.10455003026448245No Hit
CCCTAGCAGTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGG16140.10448529340363756No Hit
ATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTC16080.10409687223856827No Hit
GAATAGAGTGCAGCACTTCTGGTACATTTGTTCATCCTCAAGAATTCCCC15960.1033200299084297No Hit
GGCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCC15880.10280213502167065No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAGC100.0070914933144.99821145
GCCGCTT100.0070914933144.99821145
ATGAGCG100.0070914933144.99821145
GACTTAA301.1020711E-7120.831856145
GCAGGTA17650.0112.5809862
GGTACTG18800.0104.537155
AGTAGAA29400.0102.6103441
CAGGTAC19300.0102.204683
CCCACGC1000.0101.49875145
GTACTGA20300.097.1699756
TTTCGTT554.1654857E-1092.2716145
GAGCCCC2350.089.466995145
CCACGAC1150.081.95551145
AGGTACT25200.079.1391454
GCCCACC2750.079.08994145
AGAAACA38950.077.82664
TAGAAAC39750.076.260283
AAATATA2500.075.421045
TCTCCGC3750.075.39907145
AACACTG3300.072.520235
Produced by FastQC (version 0.11.5)