FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2239_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2239_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1959426
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG31890.1627517446435844No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC21210.10824598632456649No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGC6000.043.499573145
TCGCCAT12700.036.535072145
TACTGCG2950.034.4143455
GCGTTAA2950.034.4064379
TGCGTTA3150.032.223558
ACTGCGT3550.030.640546
GTGATCG4350.029.9997069
GTATTAT19150.029.5350111
TATTAGT17850.029.2501262
TACACCG1753.7705868E-629.0063765
TATAGCG2553.0195224E-928.4376242
TTACCGC1306.478789E-427.8893241
GCGAAAG3950.027.5376992
TAGAGGT16150.027.3850298
CTATTAG19350.026.231821
TCTCCGC5700.025.438345145
AGAGGTC17700.025.395239
TATACTG10050.025.2543055
GCACCGT3001.7053026E-824.1719826
GGATAGC6100.023.7757195
Produced by FastQC (version 0.11.5)