FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2239_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2239_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1716610
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG25420.1480825580650235No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAT11000.042.181393145
TCGCGTT800.00203712136.249638145
GCGAAAG4550.035.060722
TATTAGT17400.034.1722952
TATAGCG3200.033.9899752
CTATTAG16050.032.0769351
CGAAAGC5950.029.2485143
ACGGCGC1000.00607076529.0047786
TATACTA6050.028.7650685
TAGAGGT18600.026.115338
GCGGTAT4450.024.4422286
AGAGGTC19900.024.0449859
GTATAAT5900.023.3513031
TTATATA18900.023.0196652
TCTCCGC5700.022.894508145
TAGCGGT5151.8189894E-1221.1199864
GTCTAAC5850.021.0718481
CACGAGC3106.2499385E-721.048176145
GTAGAGG23150.020.6693387
CTATACT10550.020.619514
Produced by FastQC (version 0.11.5)