FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2238_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2238_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1278205
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT41570.3252216976150148No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG25480.1993420460724219No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC23760.1858856756154138No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA16420.12846139703725146No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG15730.12306320191205636No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC14470.11320562820517836No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT13730.1074162595201865No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT13340.10436510575377189No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT13150.10287864622654426No Hit
TTGTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTG12930.10115748256343857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG3000.045.925192
TATAGCG1453.8380676E-1045.0083542
AGCGAAA2850.043.2536431
GGATAGC2650.041.0453535
GTATAGG8900.040.73791
GTTACGT907.475427E-540.2852551
CGAAAGC3600.038.2709923
GCGGTAT1854.1763997E-935.2768176
TTACGTG1051.8519831E-434.5302162
ACCGTGT5800.033.7496648
AGCACCG5500.032.9606635
ATAGGTG11500.032.7886963
GCACCGT5550.032.663726
ACCGCAC900.003628783232.22198
GATATAT7150.031.4394021
ACATGGT6250.031.3258135
ACCGTTA950.00472801430.526018
TTATATA27400.028.5819452
GTATATC2553.0195224E-928.4366511
TAGGTGG13100.028.230434