FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2238_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2238_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424098
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT35670.25047433533366387No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG23480.16487629362586No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC23120.16234837770996097No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG22140.15546682882779134No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA21920.15392199132363082No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT18390.12913437137050962No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA16670.11705655088343639No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG16100.11305401734992956No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC15270.10722576676605122No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG2300.050.4447982
CACGAGC1002.7351925E-643.49947145
ACATGGT7050.043.201585
GCGGTAT2700.042.9730076
GCGAAAG3600.042.3000642
TCTCCGC4500.040.277287145
GGTACTG3700.039.198355
AAGGCGC2300.037.82563145
GGATAGC5650.037.2210855
AAGCGGC800.002034920536.25724
ACGGGTC800.00203667536.250838
CGAAAGC4250.035.8306433
GTACTGC2750.034.2795334
GTTGACA9000.033.8400541
TCGCCAT7900.033.037575145
TAGAGGT8150.032.914878
CTATAGG5100.032.702571
CGGTATC3550.032.6768077
AGAGGTC8600.032.0367479
GTACAGC3250.031.238076