FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2238_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2238_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2013845
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG6350.050.243332
CGAAAGC6450.049.464363
AGCGAAA6450.049.463131
CGCGCCA2300.044.1301049
ACGCGCC2600.041.827658
GACGCGC1859.640644E-1139.189877
AACGCGC951.0291575E-438.1585547
GTTACGT2206.220944E-1032.9583851
CACGAGC4200.027.61884145
CGCCATA1050.007710559827.61884145
CTATACT7700.022.60064
TCTACGG1952.6135173E-422.310852
AAACGCG1650.002599631721.9728056
TTACGTG3355.4975317E-821.6448522
CACCGTT2053.6677954E-421.219887
TATACTG11750.020.9816915
GCACCGT6550.019.9264836
TCTCCGC7650.019.901812145
TAAGACG6300.019.5662574
GGACCGT3852.3844768E-718.8338346
Produced by FastQC (version 0.11.5)