FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2238_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2238_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1593111
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG4500.046.7319832
AGCGAAA5650.035.935581
GTATATA7350.030.5836581
CGAAAGC7150.027.3842033
TATACTG6600.026.3699725
CACGAGC5150.025.33953145
ATCGTCG1500.001494209224.1679238
ACGACTC2502.2758722E-623.2055765
CTCCGAC1902.1941238E-422.894487145
CCACGAC3655.691618E-921.84908145
ATGCCGG2353.7112677E-521.59551145
GTACCTA7600.020.9918846
TAGGACG2857.0875576E-620.3557685
CGCATAC1800.00430218520.143733
TCTCCGC5153.8198777E-1119.708525145
TATAGCG1850.00504207119.5986882
GTATTAT13800.019.4418561
GTCGGCC5605.456968E-1219.419432145
ACTCCGA3852.3827852E-718.834511
TAGTACC9750.018.5942124
Produced by FastQC (version 0.11.5)