FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2238_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2238_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1893274
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT20320.10732730708814466No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT3250.049.0855561
TTACGTG3700.045.0754972
CACGAGC5100.041.22512145
ACTCCGA5300.032.8359681
CCGAGGT5700.031.8038434
GTTGACA14900.031.6330831
AGACCGC3250.031.230492145
TAGTCGG2601.10958354E-1030.673618
CGAGGTG6000.030.2136485
ATCTATG11100.029.3970641
TCTCCGC8900.028.510983145
GATAGCG4850.028.40181145
ACATGGT16700.027.7893185
CTCCGAC3153.45608E-1127.618801145
TCCGAGG7650.025.592743
CCGACTA4300.025.2951494
CCACGAC3451.09139364E-1025.217167145
GTCGAAG2051.2892975E-524.7604563
AGTCGAA2051.2892975E-524.7604562
GTATTAT23350.024.222681
Produced by FastQC (version 0.11.5)