FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2238_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2238_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1774266
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC26200.14766669710178743No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGC450.00963881848.3352857
GGTTATA4950.033.6948781
CTCCGAC3100.032.741413145
TCTCCGC6700.031.380096145
GTTATAT4750.030.5335712
GTTACGT2752.1282176E-1029.0068951
CGAAGCG1255.1533605E-429.001999
GAATAAC11300.026.9533081
CCACGAC5000.026.099583145
GTTGACA13350.025.5304151
TATAGAT4950.024.904912
GTATAAG3501.2914825E-1024.8630521
AGCGAAA6550.024.3569361
ATCTATG8400.024.1724131
GCGTTAA1500.001494130824.1683279
GTACCGC2502.2756085E-623.206176
TTACGTG3503.4960976E-922.7911322
AACCGCG2302.7431575E-522.0661097
AACCGAT2003.0993644E-421.755172
GTCGAAG2003.0993644E-421.755173
Produced by FastQC (version 0.11.5)