FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2238_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2238_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227451
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGC651.3980389E-766.924987
ACGCGCC1650.061.5194058
CGAAGCG400.00604955754.378769
TCGCGTT450.00964012548.332737145
GACGCGC1255.1968527E-946.4013147
CGCGCCA2200.046.1395539
TGATCCG1601.0095391E-940.784079
AAACGCG1209.550415E-636.2584156
AGCGAAA4000.036.256941
GCGAAAG4100.035.372622
ATCGTAG950.004723288630.5321561
CTCCGAC1902.0774678E-730.525938145
AAGGTCG1952.606539E-729.7456479
GTATTAG2951.4551915E-1129.497171
TATACTG11450.028.5000635
CGAAAGC5200.027.8899523
CGCTAAC1409.984737E-425.8978121
GATAGCG2808.201823E-925.892536145
GTTACGT2259.095147E-725.7827111
GGTCGTT2051.2902923E-524.75687
Produced by FastQC (version 0.11.5)