FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2238_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2238_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634426
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG450.00963252748.3431051
GACGCGC752.5500978E-548.337197
AGCGAAA5250.042.8181761
GCGAAAG6500.035.701712
CGAAAGC6400.035.1264343
CTCCGAC2601.10958354E-1030.672707145
GTGATCG1952.609304E-729.743239
CGCACCG1000.006067759429.0076375
CTATCGA1050.007699958527.626323
TATACTG12900.025.8595245
GGTCGTT4151.8189894E-1224.4597827
GTCGTTT4201.8189894E-1224.1678548
ATGCCGG4201.8189894E-1224.166376145
AGACGGC7650.023.692526145
CTAATAC4154.3655746E-1122.7168253
AGGGCGT1650.002597584621.9754836
GTAGAAA16900.021.884462
CCTTATA13050.021.6723732
AAGGTCG5151.8189894E-1221.1220685
TCTCCGC6950.020.86306145
Produced by FastQC (version 0.11.5)