FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2238_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2238_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307487
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC450.00964021848.3327988
CGAAGCG803.729631E-545.324135
AGCGAAA4450.044.0000531
GACGCGC907.484196E-540.2773327
GCGAAAG4950.039.5556072
GACCGGC3000.036.249596145
CGAAAGC5850.033.4701273
GGTGCGA1000.00607546128.9996788
GCACCGT9150.028.5319126
CGGAGTA2903.947207E-1027.507064
AGCACCG9050.027.244565
CGCGCCA1658.421152E-526.3633429
CTCCGAC2601.1454358E-725.095875145
GTATTAG3251.4824764E-924.544761
CCGACTA3301.7680577E-924.1728694
ACCGTGT10300.023.9317748
CACCGTG12350.023.4815227
TAGCACC12050.023.4707534
GTTATGT9900.023.440361
GTACTAC1550.001806086923.3930991
Produced by FastQC (version 0.11.5)