FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2238_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2238_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861207
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG301.5993897E-596.66597145
TAGACCG553.218865E-452.726899
CACGAGC3700.037.22946145
CGAAAGC3450.031.5270043
AGCGAAA3350.030.303571
GCGAAAG3450.029.4252032
ACGGCGT1100.00966298526.368046
TCTCCGC5000.026.099812145
AGGACGT1409.983729E-425.897185
TATACTG4900.023.6774225
AAGACCG1952.6144626E-422.3075317
TATATAG2704.4372464E-621.485072
CCGCTTG1700.00309257921.3233769
AGACCGT2104.3136708E-420.7141388
GATATAT4601.7644197E-1020.4925521
GTATACT2155.049662E-420.2359374
AGACGGT1800.00430677320.138744145
GTATAAG3259.810719E-720.0802751
ATTATAT6600.018.677361
TACACAG8350.018.2365785
Produced by FastQC (version 0.11.5)