FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2238_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2238_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121606
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG7100.044.940592
ACGCGCC5850.040.899758
GACGCGC5750.036.5664257
CGCGCCA6400.036.2520529
AGCGAAA8950.035.6511961
CGAAAGC9700.035.1374783
AGACGCG5950.034.1268166
TGATCCG5200.032.0691229
CGTAGAC1000.00606869929.007113
GTATATA9550.025.8178461
CACGAGC3652.2009772E-1023.83528145
ACCGTGT10200.022.7463868
GCACCGT9950.022.594016
GAATAAC14350.022.2354151
TATACCG2353.7046935E-521.601045
TATAATA13550.021.4074612
GTAGCCG3406.43613E-821.3247369
CACCGTG13100.021.0312277
GTATTAA36250.021.0051481
TAAGACG10900.020.624324
Produced by FastQC (version 0.11.5)