FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2238_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2238_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567259
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG16600.10591740101667944No Hit
GTATTAATTGATATGCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCT15830.10100436494542382No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGC1650.052.729787
TGATCCG4450.042.3602149
TCTCCGC5400.041.619694145
GCGAAAG6000.039.8883482
CGCGCCA6150.038.9030469
GTCGTAG750.001481204638.679611
AAACGCG2300.037.839966
ACGCGCC6750.035.4449968
GTGATCG2900.035.0005469
CGTAGAC1051.8505512E-434.5353663
AGCGAAA6900.033.6344451
CGAAAGC6950.033.3924673
ATGCCGG2101.4411853E-831.070923145
GAATAAC7250.029.0097061
GACGCGC5300.028.727787
CAAACGC3453.6379788E-1227.3288575
CCGAATA1658.4206695E-526.3640469
CTCGCGG1701.0317724E-425.58782145
GTCATAG8150.024.9163132
GTCCTAT8950.023.4994851
Produced by FastQC (version 0.11.5)