FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2237_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2237_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112849
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATATTCAGAGTGCTAAATGAGAAAACTAACCACCCAAGAGTACTTTA25200.11927023653843696No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6500.075.8648151
GCGAAAG8650.057.0082442
CCGACGA450.00963124548.3452222
CGAAAGC10400.046.718223
TCTCGTT1903.6379788E-1241.973083145
ACCGTAC1451.9254003E-840.009841
TATATCG2500.037.711066
ATCGAAT2700.034.9085589
TATCGAA2850.033.0712668
ATATCGA3050.030.9033897
GACCGGC5050.028.712458145
GTATAAG11650.026.1437681
TAGGTCG1450.001224968125.0067425
AACCGCG2051.2905832E-524.7574987
CCTATCG4403.6379788E-1223.06785145
GCAGCTA9100.022.3084288
GATATAT26350.021.4663811
GATATCG3809.05311E-920.9875359
TATATAC15550.020.5195183
AGAACCG6850.020.1149125
Produced by FastQC (version 0.11.5)