FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2237_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2237_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561389
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5500.079.10051
GCGAAAG6200.070.16982
TAGGTCG657.3334726E-444.6207925
CGAAAGC10500.042.8147163
ACCGTTC1255.1553553E-428.9998028
CACGAGC4600.028.369371145
GTATAGG5150.028.1587561
CAATCCG1358.085078E-426.8516679
GTGTAGG6450.025.8558481
GGTCTAC2001.0649663E-525.3780771
AGTAGAC8300.024.4607963
GTATATA8850.023.7599411
TATAATA14200.022.9781362
GTATAGC5950.021.9354321
GTGTAAG18400.021.2797551
GTAGAGT8900.021.1823433
GAGCGAA2404.360822E-521.1483971
GTAAGAG17150.021.1395873
CTTGTAC2455.1115872E-520.7167973
TCTCCGC5700.020.350739145
Produced by FastQC (version 0.11.5)