FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2237_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2237_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1822332
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5500.085.708651
GCGAAAG7700.061.222142
CGAAAGC8400.056.983683
GTAACGC450.00962744148.3497923
CGATATA1252.7133137E-740.6138272
ATACCGT1406.595983E-736.2623446
TATACGG2008.929419E-932.636115
TATACAG8900.030.1507135
CGTAAGG3251.8189894E-1229.0098762
TATACCG1753.766856E-629.0098765
CCTATAT3301.8189894E-1228.569551
CGTAGAC1804.690308E-628.2040463
TAGTACC9400.027.0038724
AACCGCG2156.128648E-726.9770457
ATTAGCG1358.0847496E-426.8521528
TCTCCGC7900.026.613491145
CTAGCGC1658.402816E-526.3726163
TACCGTA2001.0660035E-525.3752867
GCGATAT2301.1009415E-625.2252881
TAACGTC1751.2561843E-424.8574227
Produced by FastQC (version 0.11.5)