FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2237_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2237_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1878156
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG8100.056.3975262
AGCGAAA9000.050.757771
CGAAAGC10700.043.3711243
CACGAGC3250.033.461315145
GCGTCGA1907.1601808E-626.7103527
TAACGTA1100.00966648826.3676765
TAGTACG1100.00966648826.3676764
TAATCGT1100.00967401726.3634629
AGACCGT4750.024.4247936
GTAACGC5350.023.040913
TAACGCA6000.022.961854
CCTACAC7950.022.8022353
TAATACT12150.022.0815724
TATACTA4951.8189894E-1221.973065
GCGTTAC6400.021.5267331
GTAATAC8850.021.3026963
CGTCGAG2404.3657277E-521.1456938
TATACGA4201.2678356E-920.7174571
GTGATCG3851.0528311E-820.714159
CCGTTTG3851.0528311E-820.714159
Produced by FastQC (version 0.11.5)