FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2237_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2237_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1869717
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC20700.11071194196768815No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6250.060.3321271
GCGAAAG6300.059.85332
ATAACGC951.0291503E-438.158428
CGAAAGC10300.038.0183683
TAACGCT1001.3913611E-436.252449
ACCGTAC1050.007702578327.6246011
GTGATCG2101.5551981E-524.1682939
TAGTACC10500.023.4815374
GTCCTAT17550.022.7253671
GTGTAGG10600.022.5752931
AGCCGAC4256.184564E-1122.1773727
TAGGCCG2003.0996578E-421.7549555
CTCCGAC3004.559879E-721.74972145
TACTGCG4358.367351E-1121.6675457
CACACTG23350.021.4288645
ACACTGG29800.021.4142746
CTGCGTA4801.4551915E-1121.1472579
ATCGCGG2455.1175666E-520.714018145
ACTGGGC23800.020.4099458
GGGTTGA19650.020.2972436
Produced by FastQC (version 0.11.5)