FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2237_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2237_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1613049
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC16200.10043092305317446No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5200.071.122671
GCGAAAG6500.056.8981442
CGAAAGC8850.042.6089973
CTCCGAC2054.0421037E-728.292393145
AGGTTCG1050.00771021527.6187658
TCTCCGC8200.026.524118145
TTATACG1100.00966210626.3699054
CCGTAAC1100.00967379426.3633659
AGACGGC7500.026.09973145
TCTCGTT4550.025.494246145
GTATAAG3301.7698767E-924.1724131
GTATATA5100.022.7505071
CACCGTA2404.3653592E-521.1456177
GTACCTA6750.020.412266
GACTCCG5353.6379788E-1220.326893145
GTACTAG2505.966482E-520.3048251
TACGGCC1850.00504125819.5992554
TAGTACT3401.5125479E-619.195744
GGGTACT2659.354946E-519.1507457
TATAACG4553.426976E-919.1254255
Produced by FastQC (version 0.11.5)