FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2237_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2237_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027534
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC3000.041.089073
GCGAAAG3400.038.387712
ACCGTAC1501.133556E-633.8347668
AGCGAAA3550.032.679031
ACCGTGC1658.416371E-526.3647528
ATAGCGT1409.99231E-425.8939518
TCTCGTT4001.8189894E-1225.374838145
CCGTACA2351.3305726E-624.6806959
TCTCCGC5050.024.405783145
GTATAGA9350.022.4886761
CGTATGG4659.094947E-1221.827818145
GTATATA8700.021.6686381
TATAATA10900.021.2873742
GTATAAG2806.0857437E-620.7161711
GTCTAAG4652.055458E-1020.270661
GTCCTAT6800.020.2591951
GAATAAC6800.020.2591951
ATGCCGG4301.7007551E-920.23243145
TAGACAG8250.020.2149435
AGAACCG1800.00430367120.14175
Produced by FastQC (version 0.11.5)