FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2237_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2237_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1696922
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAT300.00193579872.501689
TACCGCG450.00963940348.3344547
GTGATCG2650.035.5668649
TGACGTA1051.8527237E-434.5286755
AGCGAAA5850.033.465271
GCGAAAG6100.032.0937462
CTCCGAC2456.444316E-826.632486145
TCTCCGC8050.026.117847145
CGAAAGC7950.024.625393
TCTCGTT4800.024.166515145
GCGTTAC2003.101932E-421.7524261
TATACTG9950.021.1336355
GAATAAC10900.020.6215671
TACTCCG1950.0068415618.5901749
GCAATCG6301.8189894E-1218.412582145
TATAACG2400.001059121818.1275565
CGTTACA3201.9273688E-518.1270222
TAACACG2400.001059331318.1270224
TAGTACC6001.8189894E-1118.1270224
ACGTCCG2400.001059960118.125429
Produced by FastQC (version 0.11.5)