FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2237_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2237_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2323682
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC38970.16770797381053001No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG28220.1214451891437813No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT28010.12054145102470992No Hit
TTAGTAGACAGCTGAACAGTCAGATGTCTGTTTCTTTAGAATTCAATCTT27310.11752899062780536No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA26050.11210656191337712No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG23680.10190723171242883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC2200.056.02193145
GTTGACA24100.048.1424561
GGTCCTA29800.041.8477449
GCGAAAG4450.040.7385252
ACATGGT28300.040.2297975
CATGGTC28300.039.974426
ATGGTCC32350.038.5499087
CACGAGC3600.038.263344145
TATACGG951.0279635E-438.1663865
GACATGG30550.037.0287174
TTGACAT33200.034.291532
TGACATG33750.033.7327043
TGGTCCT37200.033.7180948
GCGTTCC7150.031.4398841
TATAATA23150.031.0105512
GCGTTAA2202.2659151E-829.6605839
CGAAAGC6250.029.0058293
AGTACGA1000.00607651428.999586145
GGTTATA13250.027.3639891
GTATAAT13100.027.1237721
Produced by FastQC (version 0.11.5)