FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2237_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2237_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750382
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC19720.11266112197223235No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG4450.043.9959952
CACGAGC6350.039.960346145
CGAAAGC7050.027.770523
CTCCGAC2654.5638444E-927.358295145
GAATAAC9550.027.3343331
AGCGAAA7500.026.104291
TATAATA11750.025.9191512
TACTCCG1409.988014E-425.8971125
TCTCCGC7200.025.173433145
TATACTG11650.023.6519575
TAATACA21350.023.4347634
AGACGCG3803.6379788E-1022.894573145
ATAATAC16900.020.59513
CGTTTAT3208.4647945E-720.3939761
ATAACAG15250.019.9704933
GTATAAT8100.019.6945931
TTAGACC7000.019.6818054
TCTATAC25000.018.563053
GTTCTAT27100.017.92731
GTATTAT14600.017.879651
Produced by FastQC (version 0.11.5)