FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2237_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2237_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031457
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC22680.1116440072322476No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG4550.035.054466145
AGCGAAA7400.033.319381
GCGAAAG8350.032.1340142
TACCGTT2101.4408215E-831.0725367
TCTCCGC7200.029.200993145
GTCCTAT17700.027.8602711
TCTCGTT6450.026.976377145
CGAAAGC9550.026.5775153
CACGAGC5750.026.477901145
TACACCG2259.0928916E-725.7850445
CGTAGAC1701.0300036E-425.5948183
GAATAAC15700.023.095111
TCCTATG22200.021.5595992
TATAATA13750.021.0963342
GTATAAT9350.020.9412141
GTATAAG9750.020.8258691
ATACCGT2806.0792245E-620.7206346
GTAATCG2505.9790116E-520.299725145
TACGCCG1800.004301552720.1445665
GATAGCG2205.9067796E-419.772459145
Produced by FastQC (version 0.11.5)