FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2237_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2237_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479441
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5200.043.2252662
CGAAAGC5850.038.422463
AGCGAAA6100.036.8452761
ACCGTAC1209.562933E-636.2522248
CACCGTA1504.3982764E-529.0017767
CCTACAC6250.024.3623163
CACGAGC4500.024.166515145
TCTATAC15750.022.097343
CTATACC17450.021.6066384
TATTACG1700.003091781421.3255582
GTCCTAT12350.021.1341841
GTATAAT5850.021.0689561
GTGATCG2806.0938546E-620.7141559
CCGTACA2806.0938546E-620.7141559
GAATAAC8150.020.4606861
TATACCA18500.019.9883885
GTATATA6600.019.7732721
ATAATAC12050.019.2549443
TATAATA11300.019.2496192
GTATTAT12800.018.691921
Produced by FastQC (version 0.11.5)