FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2235_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2235_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869006
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAATACAGTGCAGATAAAGGCAGGTATGAACATTGGTGCTGGCCATT10440.12013726027208096No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC451.09066605E-896.677061
CCGTACA1007.185008E-1058.006242
GACGCGC1402.728484E-1046.6067927
AGCGAAA3050.038.0368771
TGATCCG1354.9706796E-737.592319
CCTAATA5200.036.25392
GCGAAAG3250.035.6961442
CTAATAC7000.035.2180753
GCAAACG850.002740343334.1213154
AGACGCG2008.938514E-932.628516
CGCGCCA2900.032.4997569
GTATAAC2301.0022632E-931.5251271
ACGCGCC3000.031.416438
GTGATCG1204.0577928E-430.2081059
CGAAAGC3850.030.1331123
GACGGGT1050.007708186727.618848
CGGACAG1409.987263E-425.8956435
TATACTG3551.546141E-1024.5096785
ACAGTGC9550.024.293018
ATGCGCC1801.5201398E-424.166485145
Produced by FastQC (version 0.11.5)