FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2235_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2235_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1586011
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG4650.056.139132
AGCGAAA5350.047.438441
CGCGCCA2600.039.0393269
GACGCGC2250.038.6675227
CGAAAGC9200.029.9510383
ACGCGCC4250.027.2947228
ATGATCG1658.422161E-526.36339145
CTCCGAC2507.8374796E-826.099756145
AGACGCG3251.4824764E-924.5436486
TCTATAC15750.023.9408133
TAGCCCG1851.8287031E-423.5184845
ATACCGT2202.2262293E-523.0730576
TAAGACG3803.619789E-1022.8995764
TACCGTG2252.6523549E-522.5560557
GTATAAG5600.022.0128861
TATACAG9000.020.1431455
CTATACC17750.020.0183144
TTTATAC10200.019.9055393
GTATTAG5254.9112714E-1119.3368111
GTCTGCG1900.00588862219.079379
Produced by FastQC (version 0.11.5)