FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2235_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2235_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1370147
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG4100.038.9051632
AGCGAAA4150.036.6893121
CGAAAGC5650.028.2320633
TAGCGCG2156.1275387E-726.976662145
TAGTACC10300.026.7494434
GTACCTA9450.022.2502336
AATAGTA12400.021.6345772
AAGCGGT3406.431037E-821.3250226
GATACCG2053.665665E-421.2209975
CACGAGC3901.2205419E-820.448654145
AGCGGTC3551.0154508E-720.4224727
AGTACCT13500.020.4088345
GATATCG2505.976705E-520.2999389
TAGTACT3951.4118996E-820.1912864
GTATAAG4351.9626896E-920.00141
GTTACGT2556.956143E-519.9033531
GCGCTCC5900.019.660957145
TAACGTG2608.0757534E-519.5205965
GAATAGT12650.019.487531
TATATAG4904.129106E-1019.236045
Produced by FastQC (version 0.11.5)