FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2235_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2235_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718325
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATCG2551.8189894E-1234.117188145
TCTCCGC8400.032.79718145
AGCGAAA4250.029.008051
CACGAGC7350.028.605059145
GTCCTAT16250.024.0989971
CGAAAGC5300.023.2618483
GCGAAAG5950.023.1583632
TAGTACT5100.022.7520734
GTTATAC4751.2732926E-1121.3749753
TATAATA12600.021.2962132
GCGTACA2104.3069466E-420.720642
CTCCGAC3301.1391003E-619.77246145
TAGTACC9750.019.3392644
GGTTATA5305.638867E-1119.156261
GTATTAT19800.019.045691
CCGACTA2751.2399403E-418.4602054
TCCTATG19700.018.406662
TGTTATA8350.018.2391262
TAGACAG14150.017.9383645
ATACCGC2450.001214906617.7605486
Produced by FastQC (version 0.11.5)