FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2235_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2235_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424653
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA14540.10205994021000202No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6000.059.2158051
GCGAAAG6300.056.397982
CGCTATA657.3318125E-444.6225782
TAAGACG3750.044.473844
ACGCGCC3600.044.31278
CGCGCCA3600.044.3111429
TGATCCG2350.043.1969389
CGAAAGC8400.042.2984853
GACGCGC3650.041.7190557
AGACGCG4900.031.0797126
GGTACTG6050.027.5673965
GGATAGC4800.027.1928375
GTCGAAC1409.987279E-425.8970323
TACGAAC1409.997513E-425.892488145
TATACTG14700.025.6512935
GTACTGA7150.025.356416
CACGAGC4600.025.21703145
ATAAGAC11050.024.9361483
TACTGAT7650.024.6444997
GTATGGA7200.024.1697141
Produced by FastQC (version 0.11.5)