FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2235_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2235_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1451507
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA20330.14006132936320664No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT18890.13014060559129237No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG16560.11408832337701437No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA15780.1087145980005608No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5500.068.558921
GACGCGC2300.059.8927577
GCGAAAG6550.058.6776732
CGCGCCA3050.052.2945489
ACGCGCC3100.051.4528588
AACGCGC450.00963908548.3345037
CGAAAGC8150.048.0479053
GAGCGAA1602.0008883E-1145.3214041
AGACGCG3200.043.0568166
ATGCCGG2950.036.86403145
AAGGTCG4300.035.4066169
AGTAGAA20150.034.9076041
GTAGAAA20100.034.6348652
TATAGCG1352.1394824E-532.2296682
TAAGACG4950.032.2296644
TATACTG15350.031.6522625
TAGAAAC22250.031.6140423
CCTTATA14800.029.3986822
CCCTTAT14800.028.9077071
ACAAGGC17500.026.9292268
Produced by FastQC (version 0.11.5)