FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2235_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2235_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561233
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGC1450.055.0061267
TACATCG2800.041.42788145
ACGCGCC2350.040.1108478
CGCGCCA2200.039.549869
GTTACGT2250.038.6759261
GTGATCG2450.038.473679
CTCCGAC1604.59313E-836.249393145
AGACGGC7300.035.752827145
AGACGCG2551.8189894E-1234.1290976
CACGAGC4400.032.95399145
GCGAAAG5300.032.838052
CGAAAGC5650.030.8038353
TAATACG1753.7693662E-629.0069454
ACGGGTA1000.00607218429.0032278
TTACGTG3153.45608E-1127.6256642
AGCGAAA6350.027.4081361
TCTCGTT4051.8189894E-1225.061308145
TCTCCGC5750.023.95612145
CCACGAC3702.6011548E-1023.513119145
TAAGACG4053.274181E-1123.2771784
Produced by FastQC (version 0.11.5)