FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2235_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2235_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514417
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG2400.048.33298145
GCGAAAG4650.040.5426942
AGCGAAA5200.036.2533261
GTTACGT2052.910383E-1035.36911
CACGAGC5200.033.461296145
CGAAAGC5900.033.1821063
AACGCGC1000.006073686329.0017057
GGTCGTT1555.5000615E-528.0661667
CGTAAGG1050.00770499127.6224942
TTACGTG2654.5565685E-927.3619042
AAGGTCG2156.1214087E-726.980115
CCACGAC4050.026.851658145
GTATTAT14500.026.0023861
TATACTG9600.024.1696835
ACGACTC2252.6501508E-522.558375
GTATATA7750.022.4536721
TAGATCG2603.2061707E-622.30753145
TACGTGT3304.6877176E-821.9724393
GACGACT2704.442547E-621.4841614
CTCCGAC3106.2487743E-721.048233145
Produced by FastQC (version 0.11.5)