FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2235_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2235_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812093
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG21730.11991658264780009No Hit
GGATCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTA21390.118040299256164No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC21130.11660549430961877No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG19550.10788629501907462No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGC3450.052.53857
CGCGCCA4150.052.409019
ACGCGCC4200.051.7879528
CCGACTA3450.039.9369744
GTTGACA13950.039.5053631
TAAGACG4900.036.998544
AGACGCG5250.034.531976
ACATGGT16250.033.915715
AACGCGC1102.4363093E-432.9559677
TAGTCGG1352.1425238E-532.2236148
GTTACGT2755.456968E-1231.6420971
GATAGCG3100.030.402863145
CGCTTAT1453.4863147E-530.0070926
TCTCCGC4650.029.6233145
TTACGTG2951.4551915E-1129.4984972
CACGAGC2951.4551915E-1129.49117145
GTACTGC7050.028.8011344
GACATGG19250.028.6301444
GCGAAAG5100.028.4380952
CATGGTC19500.028.2630886
Produced by FastQC (version 0.11.5)