FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2235_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2235_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1582445
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC15950.10079339250337295No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCA3450.046.231289
GCGAAAG6650.045.803352
AGCGAAA6950.042.7827451
ACGCGCC3950.040.3792238
CGAAAGC7850.037.8777123
GACGCGC3450.035.724177
AACGCGC1102.4369743E-432.9541177
CACGAGC6400.032.85113145
GATAGCG3450.031.521328145
CTCCGAC2558.913048E-1131.274101145
TCTCCGC5600.031.071022145
GTTACGT1606.8165355E-527.1957381
TAGATCG1606.8312176E-527.187143145
AAACGCG1358.070511E-426.8599876
TGATCCG3800.026.7101759
CCACGAC3306.184564E-1126.36329145
ATGCCGG2451.9134714E-623.67316145
GTATATA7850.023.0961671
AGACGCG5400.022.830996
AAGACGC5450.022.621535
Produced by FastQC (version 0.11.5)