FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2234_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2234_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1906344
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT21320.1118371080980138No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC19900.10438829508210482No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGT901.3242843E-648.332969
CTCCGAC2700.045.647797145
GCGACTA803.732637E-545.319284
GTTACGT3950.044.057221
TTACGTG4550.038.2474822
AGTCGAA2252.0008883E-1135.4497452
AGCGAAA4300.035.4122731
CAGTGCA10100.034.46062
GTCGAAG2603.6379788E-1233.4665453
CGAAGCG900.003626391832.2270435
AAGTCGA2507.094059E-1131.9047721
TGAGCGC8900.031.769415145
CCGACTA5300.031.466974
TATACTG7400.030.3761675
ACGTGTT6000.029.0043374
GCGGACC1255.155806E-428.9997739
CCACGAC3301.8189894E-1228.560383145
AGTCTAT5950.028.0294041
CACGAGC5250.027.618834145
GGGTTAC2903.947207E-1027.5041141
Produced by FastQC (version 0.11.5)