FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2234_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2234_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319012
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC14260.10811122264240203No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC1700.055.440918145
GCTACTA2650.043.773389
GTTACGT2100.041.433091
CCGACTA2550.036.9648134
AGCGAAA3250.033.4651871
CACGAGC4300.032.034733145
CAGTGCA7150.030.4228972
GATATAC2252.8156137E-829.0031623
TTACGTG3001.8189894E-1129.0031622
TATACTG6700.028.1373965
AGTCGAA1555.49765E-528.0675742
GCGAAAG4400.028.0144161
GTATTAG2654.5565685E-927.3614751
TAGTACC8300.027.0812644
TCGACTG1100.00967290426.363519
CGAAAGC4700.026.2262652
GTACCTA9100.025.4972846
GTAGAAA8250.025.4876272
GTATTAT15600.025.098891
GCCGTCG1450.001226684524.999882145
Produced by FastQC (version 0.11.5)