FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2233_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2233_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGGGGTGAGGGACAAAGGGAGAGAGAGAGAATCCCAAGCAGACTCC12.941176470588235No Hit
AGTTTATAGTGATACAGGCCTAACTCAAGAAACAAGAAACGTCTCAAATC12.941176470588235No Hit
CACTCAGCGCGCATCCTGCTGGAGGATACCCACTCTCCCTCTGACCCTAC12.941176470588235No Hit
ATTATGTGTTTAGTGGTTGTTATTGTCATAGTGTGTCCTGGTAATTGAAC12.941176470588235No Hit
ATCTTAGTAGGTTGTATGTTTCTGTAAATGTATACTATTCTTTTGGATTA12.941176470588235No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT12.941176470588235No Hit
CTACAAAATTAGTAGTAATTCTAATAAACAGACAGGAGAGCCGAAGAGCA12.941176470588235No Hit
ATTCTGCATTATATGCCCATACGTCTTGTATTTGGTCATCAATCTTATTA12.941176470588235No Hit
CTCCAGGATATTGAAAATGAGGATAAAATCCCAAAAACAAAGAACATGAA12.941176470588235No Hit
GAGGGGGATGCAGGCTCTCTGCTGAGTAGCGAGCCGGATGCAGGGCTCGT12.941176470588235No Hit
TAGGGACGGGGCTACATGGGCTTCAAGAGCCTGAGAGCCCATCTCTCCAG12.941176470588235No Hit
CAAGTATACTGTATATACAAAAACAAGGATAGTACAAAACAAACTAGAGA12.941176470588235No Hit
GTTAATGATGTCCCTGTGTGAAATTATAGATGCTCGTCCCCGCTGTCCAC12.941176470588235No Hit
GTAGCAGCGCTATCAAATGCTTTTTCTACATCTGTTGACATGGTCCTATG12.941176470588235No Hit
GAATGAGGGGATGTCCCAGGCTTGTTGCACACAGCATTCCATTATGCTCT12.941176470588235No Hit
AACCTGAACAACCAACCACAATAAGCGTGGATGCTTCTGGAGGATACCCT12.941176470588235No Hit
CCCAAACCCCAGACCTCATTTCTCAATACTTAATATAATTTTGGCAAAAA12.941176470588235No Hit
GTTCATGGCTGCAAAGCTAACAGATGATCTTCTGATTTTCAGTCCAGCGG12.941176470588235No Hit
GAGCATTCCTTCCTCTTGTTTGTCTGGCAGAGTTGGAGAAGGATTGGTAT12.941176470588235No Hit
GAGACAAACTGACAGACTGACCAAAGAGGAAGCAGCAGACGGCAGTAGAA12.941176470588235No Hit
AAGGGAGAGAGAGAGAATCCCAAGCAGACTCCCCGCTGAATGTGGAGCCC12.941176470588235No Hit
GGTCATAGGAAAGATATTTCAATTCCCAAAAGTTAAAAGAACATATCAAG12.941176470588235No Hit
AGCCAGTTGTCCCAGCAACATTTATTGAATAGGGAGTCCTTTCTCTGTTG12.941176470588235No Hit
GAAAAGGCTGACTCATCAACTGGACAAGAAACTCCATCTATTCATCTTAT12.941176470588235No Hit
CATGAAATAAGAAATCAAAAACTTCCAACAACCAAAAGTCAACGACCCCA12.941176470588235No Hit
CCTGCCAGGATTGAATCCTAAAGAAACAGAAATATGAACAGACCAATGAG12.941176470588235No Hit
CCCGAAAATTGAAACAAATAAGTTCGCTGCAGCTGAATCGAGAAAACTAC12.941176470588235No Hit
CTCGCAGCCAAGCATGTGCTTCATGAAGAACTCGGTGCCTTTGGCACTGG12.941176470588235No Hit
GAGCTGAAGCCAGGTGCTAAACCAACTAAGCCACCCAGGTGTCCCCTAAA12.941176470588235No Hit
CCCGAGGGCCAGTGGGCGGGGTGAGGCAGGCGTGGAGCCTTGGGGGCCTG12.941176470588235No Hit
TCACAAAGCTGCTGGGGATGGAGCCAGACAACGAGGCCCTGATCATGCTA12.941176470588235No Hit
ACGCTAAGCTGTGATCTACAACTCATATCTCACCAGTGAATAAAGATACA12.941176470588235No Hit
ATAAGATCATCTGTTAGTTCTGCAGCCATGAACAAAGCACACCCCTGCTC12.941176470588235No Hit
AGAAAAAAAATCCAAAAACTGTAAAAAGAGCAAATAGAGCGCAATAAAAC12.941176470588235No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)