FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2233_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2233_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2326314
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC123890.5325592331903604No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT85620.36805005687108444No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA84730.36422426207296177No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA79070.33989392661523765No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG69410.29836900779516434No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT69320.29798212966951154No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT68330.2937264702873301No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA64740.2782943317196217No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA61950.26630110982438315No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC57710.24807485146029298No Hit
CATCACCCTCAGACAACCCCTTACTTGGATGGACTTGACAGATACACACA56280.24192778790825312No Hit
GTTGACATGGTCCTATGATGTTGATCCTTTGTTTTGTGAATGCGGCACAT53690.230794295181132No Hit
CTTATATGGACTTGACAGATACACACAGAACCCTCCATCCAAAAGCAGCA47990.20629201388978444No Hit
GGAGAGTTCTGTGTGTATCTGTCAAGTCCATCCAAGTAAGGGGTTGTCTG47650.20483047430398474No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA45450.1953734534546927No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCACAT43310.1861743513558359No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG38880.16713135028203416No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA38700.16635759403072845No Hit
ATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATTTG37900.1629186773582586No Hit
CTTCCCTCAATGATCCCCCATCCAGACAGAAGATCACAAAGGAAACATCA36230.1557399388044778No Hit
CCTAAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTT36200.1556109794292602No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG34710.14920599712678512No Hit
ACCTGTTACTTAGATGGACTTGACAGATACACACAGAACCCTCCATCCAA33900.1457240939959094No Hit
CCCAAATATCTGCAAACCAAATTCTACAATACGGGAAGAGGTATGATCCA33350.1433598387835864No Hit
ATACCATAGGGAACTTTAATACCTCACACTTCTCTCAATGATCCCCCATC33050.1420702450314102No Hit
CTTTAATACCTCACACTTCTCTCAATGATCCCCCATCCAGACAGAAGATC33000.14185531273938082No Hit
GAATAACAGATCAGCAAAGAGCTGGTTCTTTGAAAAGAACAAAGCTGACA32500.1397059898190872No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT31570.135708249187341No Hit
GGATCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTA31490.13536435752009401No Hit
GTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTGA30530.1312376575131302No Hit
TATATGAACAAACCTAAGAGAAGCAATCAGCACCAATACCATAGGGAACT30420.1307648064706656No Hit
CTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAAGATGGTTG29280.1258643502123961No Hit
GAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACCAAATTCTAC28590.12289828458239085No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACTCTCCATCCAA28550.12272633874876736No Hit
GCTGACAGAGTCTCAGCCAGATTCAAAGAAAGAGGACTCATCATCAGAAA27870.11980325957716799No Hit
GTTTTCTTCTTGGAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA27640.11881457103383292No Hit
GTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCATTAGTTTGAT27210.11696615332238039No Hit
CCCAACTGGTTGTTCAGTAGCATGTTTTTTAGTCTCTGTGTTTATGAATT26870.11550461373658069No Hit
TTCTACATCTGTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAA26260.11288243977382245No Hit
ATTTCCAGTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGT25880.11124895435439927No Hit
GTACCCTTTTCTTTTGGATTATCCAGCTTGTTGGGGTGTAATTGTTCATA25420.10927157726772913No Hit
GTTTACTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA25250.10854080747482928No Hit
GAGGCTGATGTTTCCGTTGTGATCTTCTGTCTGGATGGGGGATCATTGAG25030.10759510538990008No Hit
GTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTG24880.10695030851381197No Hit
GGTTATATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTATCCAGCTTG24590.10570370122004168No Hit
GATCAACAAAGAGCTGGTTCTTTGAAAAGAACAAAGCTGACAGAGTCTCA24430.1050159178855477No Hit
GTTCCAGGGGCACTTGAGAGGAGTATATTCCTTTTGCTGCTTTTGGATGG24160.10385528350858912No Hit
GCGTTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTATTC23900.10273763559003643No Hit
GAACCCTCCATCCAAAAGCAGCAAAAGGAATATACTCCTCTCAAGTGCCC23830.10243673038119533No Hit
TATATGAACAAACCTAAGAGAAGCAATCAGCACCAATATCATAGGGAACT23520.10110415017061325No Hit
TCAACAAAGAGCTGGTTCTTTGAAAAGAACAAAGCTGACAGAGTCTCAGC23440.10076025850336626No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTATA8150.078.2900161
ACATGGT43900.068.71145
GTTGACA45050.066.6326141
CAGTGCA25600.066.2775652
GCAGTGC27650.061.362361
TTGACAT49600.060.8138242
GACATGG49500.060.7915084
TATATGT10750.060.0318224
GTTACGT1450.060.0059851
AGTGCAG29650.057.2244723
CAGCAAC22850.056.7975357
CGAAGCG651.093173E-555.7771955
TGCAGCA23300.055.7053765
ATATGTT11800.055.3045045
GTGCAGC30750.054.7069934
GTTATAT11300.054.542012
GCGACTA400.00604847554.382764
CATGGTC55800.053.9279486
CTCCGAC1500.053.166256145
ATCTATG19550.052.2942161
Produced by FastQC (version 0.11.5)