FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2233_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2233_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1799974
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC73750.4097281405175852No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT41690.23161445665326277No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT32290.17939148009915698No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA30940.1718913717642588No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC27160.15089106842654396No Hit
GTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTG26180.14544654533898824No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG24160.13422416101565912No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA23980.133224146571006No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA23790.13216857576831667No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT23650.1313907867558087No Hit
CCCAAATATCTGCAAACCAAATTCTACAATACGGGAAGAGGTATGATCCA23170.12872408157006712No Hit
ATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATTTG22970.12761295440934145No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA22860.12700183447094235No Hit
CTTTAATACCTCACACTTCTCTCAATGATCCCCCATCCAGACAGAAGATC22760.12644627089057953No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA22430.12461291107538221No Hit
CATCACCCTCAGACAACCCCTTACTTGGATGGACTTGACAGATACACACA20860.1158905628636858No Hit
GTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCATTAGTTTGAT20600.11444609755474247No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG20510.11394609033241591No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG19780.10989047619576726No Hit
GGAGAGTTCTGTGTGTATCTGTCAAGTCCATCCAAGTAAGGGGTTGTCTG19760.1097793634796947No Hit
GAATAACAGATCAGCAAAGAGCTGGTTCTTTGAAAAGAACAAAGCTGACA18460.10255703693497795No Hit
TATATGAACAAACCTAAGAGAAGCAATCAGCACCAATACCATAGGGAACT18080.1004458953295992No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC1600.067.9682145
GGTTATA5950.067.0293051
CGAAGCG553.216733E-452.7371065
GGTCGTT1152.4865585E-950.4343767
GTTGACA22000.050.1002461
TATATGT8250.049.22134
ACATGGT22900.048.4478955
CACGAGC4750.047.315407145
GTTATAT8150.046.2662932
TTACCGC1201.97173E-742.299551
CAGTGCA10850.042.1046262
AAGGTCG1401.4133548E-841.4362955
ATATGTT9300.041.324915
TTGACAT27350.040.0348852
GGTCCTA39150.038.8885779
TACCCAC15600.038.580912
CAGGTCG6050.038.3467987
GACATGG29150.037.8115044
TGGTCCT40450.037.4595268
AGGTCGG6600.037.3481868
Produced by FastQC (version 0.11.5)