FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2233_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2233_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2044571
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA187260.915888956656433No Hit
GGATCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTA137620.6730996380169728No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA116440.569508224463714No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT106360.5202069284950241No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA100280.490469638863116No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG92640.45310238675986303No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG89710.4387717521181705No Hit
GCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTATTC84630.4139254640704578No Hit
GACATCTGACTGTTCAGCTGTCTACTAATGACCAGTAGAGAGATTAAATA83890.4103061228981532No Hit
GACTGTTCAGCTGTCTACTAATGACCAGTAGAGAGATTAAATAAGAAATC78530.3840903544068658No Hit
CTTATATGGACTTGACAGATACACACAGAACCCTCCATCCAAAAGCAGCA77890.38096011339298075No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG72050.3523966641412795No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA70820.34638073219271914No Hit
GCAGTGCAGCTACTAAATATGTAGAGCATATATGAACAAACCTAAGAAAA70170.34320158116299215No Hit
GTTGACATGGTCCTATGATGTTGATCCTTTGTTTTGTGAATGCGGCACAT65830.32197463428758405No Hit
GTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTGA63260.30940476021620184No Hit
GTAGCATGTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG63210.309160210136992No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT63150.3088667500419403No Hit
GAGCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTAT61600.3012856975864375No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAAAAG58520.2862214127071156No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA57130.279422920505084No Hit
AGTCTGTTCAGATGTCTGTTTCTTTAGAATTCAATCTTAGTAGGTTGTAT55100.2694941872891673No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC54620.26714650652875344No Hit
CCATCCAAGTAAGGGGTTGTCTGAGGCTGATGTTTCCATTGTGATCTTCT52930.2588807138514632No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT52560.2570710432653109No Hit
GTAACAGGTTGTCTGAGGTTGATGTTTCTGTTGTGATCTTCTGTCTGGAT51600.2523756817444833No Hit
GGATCATTCCTTCCTCTTGTTTGTCTGGCAGAGTTGGAGAAGGATTGGTA49830.24371860894045744No Hit
CCCAACTGGTTGTTCAGTAGCATGTTTTTTAGTCTCTGTGTTTATGAATT48060.23506153613643155No Hit
AGAACAATATCAGATCAACAAAGAGCCGGTTCTTTGAAAAGAACAAAGCT45490.22249166206504936No Hit
GACTGTTCAGCTGTCTACTAATGACCAGTAGAGAGCTTAAATAAGAAATC41890.20488405636194584No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC40750.1993083145559631No Hit
AGATATTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTAT40110.196178073542078No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTACCCTTTTCTTTTGGATTA39730.19431949294008377No Hit
GTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATG39200.1917272621004602No Hit
TATGCAAAGGCTCTTAAGAATGTACTATGAACAATTACACCCCAACAAGC39070.1910914318945148No Hit
GCTCTTAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAATC38370.187667730785578No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT38340.18752100073805214No Hit
GATATTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTATG38260.1871297206113165No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACTCTCCATCCAA37580.18380383953406362No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA36700.17949975813997165No Hit
GCAGTGCAGCTACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA35690.17455984653993428No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT35380.17304363604883372No Hit
GTAAGGGGTTGTCTGAGGTTGATGTTTCTGTTGTGATCTTCTGTCTGGAT33940.16600059376759232No Hit
TCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTAT33860.16560931364085668No Hit
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG33460.16365291300717852No Hit
GGAGAGTTCTGTGTGTATCTGTCAAGTCCATCCAAGTAAGGGGTTGTCTG33170.16223452254776186No Hit
GATTCAAAGAAAGAGGACTCATCATCAGAAATGAGAGAGATGTTCCCTGA32850.16066940204081934No Hit
ACACCAACAAGAATAACAGATCAACAAAGAGCTGGTTCTTTGAAAAGAAC32730.16008248185071589No Hit
GACATCTGACTGTTCAGCTGTCTACTAATGACCAGTAGAGAGCTTAAATA32370.15832172128040553No Hit
TGACTGTTCAGCTGTCTACTAATGACCAGTAGAGAGATTAAATAAGAAAT32000.15651205069425322No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCACAT31820.15563167040909806No Hit
AGTCTGTTCATATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT31440.15377308980710377No Hit
GCGTTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTATTC30260.1480017079377532No Hit
AGAACAATATCAGATCAACAAAGAGCTGGTTCTTTGAAAAGAACAAAGCT30110.1472680577001239No Hit
ATTTCCAGTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGT29900.14624094736744284No Hit
ACCACTGATATGCAAAGGCTCTTAAGAATGTACTATGAACAATTACACCC29850.14599639728823308No Hit
ACCTGTTACTTAGATGGACTTGACAGATACACACAGAACCCTCCATCCAA29710.14531165706644572No Hit
CTTGGCAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTATC29600.14477364689218425No Hit
GATCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTAT29520.14438236676544858No Hit
CATCACCCTCAGACAACCCCTTACTTGGATGGACTTGACAGATACACACA29360.14359980651197732No Hit
GCTCATAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAATC28850.14110539570403766No Hit
ACACCAACAAGAATAACAGATCAGCAAAGAGCTGGTTCTTTGAAAAGAAC28830.14100757567235375No Hit
GAGCATTCCTTCCTCTTGTTTGTCTGGCAGAGTTGGAGAAGGATTGGTAT28480.13929572511788538No Hit
ATTTCATCTCTCTACTGGTCATTAGTCTGTTCAGATGTCTTTTTCTTTAG28020.13704586438915548No Hit
GCATTCCTTCCTCTTGTTTGTCTGGCAGAGTTGGAGAAGGATTGGTATTC27950.13670349427826178No Hit
CATCTGACTGTTCAGCTGTCTACTAATGACCAGTAGAGAGATTAAATAAG27230.1331819731376411No Hit
GAATAACAGATCAGCAAAGAGCTGGTTCTTTGAAAAGAACAAAGCTGACA26820.13117666248812096No Hit
TGTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTG25610.12525855057124452No Hit
ATGACCAGTAGAGAGATTAAATAAGAAATCAAAAACTTCCAACAAGCAAA25380.1241336202068796No Hit
CTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAAGATGGTTG25210.12330214993756636No Hit
GCTGACAGAGTCTCAGCCAGATTCAAAGAAAGAGGACTCATCATCAGAAA25000.12227503960488534No Hit
GTTTACTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA24970.12212830955735947No Hit
GTACAATTATCCAACATTTATAAATATTTACTGCCCCAGCATGGAGCAAC24890.12173702943062384No Hit
GTTTTCTTCTTGGAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA24880.12168811941478189No Hit
GCCCACGAACCCAATTGGTTGTTCAGTAGAATGTTGTTTAGTCTCTGTGT24770.12115010924052037No Hit
CCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAGAA24040.11757967808405773No Hit
GCATCGTTTTACTGCATTAATTCCATTATCAGTTGTAGTGCCCTTCATTG23690.11586782752958932No Hit
TCTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCA22840.11171047618302324No Hit
GTTGCATCATTTTACTGCATTAATACCATTATCAGTTGTAGTGCCCTTCA22790.11146592610381348No Hit
GGGATCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGT22530.11019426569192266No Hit
GATACATATTTTTTAAAAAACACCAACAAGAATAACAGATCAGCAAAGAG21890.10706402467803759No Hit
ACCTACTAAGATTGAATTCTAAAGAAACAGACATCTGACTGTTCAGCTGT21870.10696620464635369No Hit
GCATCATTTTACTGCATTAATACCATTATCAGTTGTAGTGCCCTTCATTG21720.10623255440872438No Hit
GAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACCAAATTCTAC21710.10618364439288243No Hit
GATTCGTAGAATTCACAGGTGAAACCATGGGGTCCTGGACTTTTGCTTGT21660.10593909431367264No Hit
GAGGCTGATGTTTCCGTTGTGATCTTCTGTCTGGATGGGGGATCATTGAG21620.10574345425030482No Hit
CAGATTCTCAGCGAGATTCAAAGAAAGAGGACTCATCATCAGAAATGAGA21440.10486307396514966No Hit
GGCTCATAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAAT21330.10432506379088817No Hit
GTAGAATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG21020.10280885329978758No Hit
CCATCCAAGTAAGGGGTTGTCTGAGGCTGATGTTTCCGTTGTGATCTTCT20980.10261321323641977No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA20880.10212411307800023No Hit
GGTTATATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTATCCAGCTTG20780.10163501291958069No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT20750.10148828287205483No Hit
CCTAAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTT20650.1009991827136353No Hit
GGCTCTTAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAAT20640.10095027269779333No Hit
GTAGACAGCTGAACAGTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGT20500.10026553247600596No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGA203.864717E-4108.763619
ACAATAT16150.088.4627464
AGTCCGC4850.086.69916145
ATCAGAT16900.084.093369
ACATGGT32000.083.8550345
ATGCCGG3850.082.85536145
CAGTGCA52250.082.723082
ATCATTC34950.082.170273
GCTACTA18850.081.5486769
GTTGACA33050.081.188961
AGTGCAG53700.080.75953
CAGCAAC34550.080.586987
TATCAGA17900.080.205578
GTGCAGC53750.080.1446844
TGCAGCA34850.079.702765
CAGCTAC19550.078.626847
GCAGTGC55050.078.515551
GACATGG34600.077.551894
CAACTGG14850.077.160913
AGCTACT20000.076.859628
Produced by FastQC (version 0.11.5)