FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2233_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2233_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605394
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC39110.24361620885589458No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT27790.17310392339824368No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG26950.16787156299325898No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG25030.1559118820675797No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT23960.1492468515517063No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA23550.1466929613540352No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA23310.1451980012383253No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA23090.1438276211322579No Hit
ATTTCATTTATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACTTT23060.14364075111779415No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG22860.1423949510213692No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT21240.1323039702403273No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC20980.13068443011497488No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA20750.12925176000408622No Hit
GAAGAAAGTTTATAGTGATACAGGCCTAACTCAAGAAACAAGAAACATCT19770.12314733953160409No Hit
GTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTG18610.1159216989723395No Hit
GTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTGA18290.11392841881805961No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT18240.11361696879395339No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA18120.11286948873609844No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA18070.1125580387119922No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA17930.11168597864449475No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT17720.11037788854324856No Hit
GTTTACTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA16850.10495865812380015No Hit
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG16710.10408659805630269No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTACG451.1935575E-464.450122
CGGGTCG350.003566972262.142525145
GTTACGC502.0097375E-458.0051083
CTAGTAC1600.049.848143
ACATGGT18000.045.920715
ATCTATG10600.044.461461
GTTGACA18800.043.5809671
GAAAGTT11600.043.12884
AAGGCGC4500.041.888664145
CAGTGCA10750.041.817642
TCTCCGC5300.041.037514145
GGTTATA4700.040.1099171
GTATTAT17200.040.047131
CTCCGAC1901.2914825E-1038.157692145
GACGACT1553.4699042E-837.422654
GACATGG22650.036.4932824
CACGAGC4000.036.249805145
GTGCAGC12850.034.9836274
GCAGTGC12850.034.9836271
AAGTTTA18700.034.897216
Produced by FastQC (version 0.11.5)