FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2232_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2232_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372124
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT16090.11726345432337018No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG13820.10071976002168902No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTA450.00963880348.334729
GTCTACG700.001056061541.4342881
GTTACGT1951.70985E-1037.1846161
CACGAGC2850.035.613758145
TTACGTG2103.783498E-1034.5285762
TATACTG6750.033.300895
CCGAGGT3750.032.87124
ACTCCGA3850.032.0174031
CAAGCGG1652.384717E-630.761823
GATAGCG3550.030.633564145
GATATAC3100.030.4074213
GTTATGT9350.030.2448141
GTCTCGG1204.0555463E-430.21251
CAGTGCA9250.029.0040022
TTAACGC1753.776193E-628.999773145
GGTGCGA1306.483103E-427.8854148
CTAGTAC1606.823545E-527.1912543
GTACTAG1606.823545E-527.1912541
TCTCCGC4800.027.187286145
GTTGACA10450.027.061151
Produced by FastQC (version 0.11.5)