FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2232_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2232_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1384230
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGCG553.2161732E-452.7381025
CTATCGG700.001056841641.428097145
CTCCGAC2154.8748916E-1033.720543145
GGTTACG900.003626346432.226518
AGCGAAA4750.030.5292741
CACGAGC2651.382432E-1030.093994145
TCTCGTT3750.028.999666145
ATGCCGG3153.45608E-1127.618734145
GCGAAAG5300.027.3631212
CGGTAGC1358.0745993E-426.8573656
CTTCGAC1358.0763036E-426.8563963
CGAAAGC5500.026.3680953
GAGTGCG1958.748624E-626.0291027
TATACTG6250.025.525245
ACGGTAG1450.001225214525.0051335
GTTACGT1450.001225988825.0024221
TAGTACC6650.023.989024
GATAGCG3503.5051926E-922.785452145
CCGACTA2252.649029E-522.5593724
AGACGGC7450.022.382296145
Produced by FastQC (version 0.11.5)