FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2232_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2232_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1410196
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT25970.18415879778413782No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG23890.16940907505056035No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT23690.16799083248002405No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC22380.15870134364301133No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA19670.13948415681224452No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG19380.13742770508496693No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA17260.12239433383728218No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT16590.1176432212259856No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG15040.10665184130432932No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA14890.10558815937642711No Hit
ACATTCTACTGAACAACCAATTGGGTTCATGGGCAAACCAAAATAGGAAC14880.1055172472479003No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT14860.10537542299084666No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT14750.10459538957705171No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC14570.10331897126356904No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGC100.007078966145.083565
ACGCGTT502.269735E-886.99766145
GTTACGT2350.083.342921
TTACGTG2650.073.910492
ACGTGTT3100.063.1815454
TACGTGT4000.052.592793
GTTGACA13400.050.885711
GCTACTA3850.050.844599
ACATGGT14350.047.0131385
CAGTGCA10950.045.7112582
AGTGCAG12000.042.3160363
AGTCCGC2400.042.290527145
TAGTCGG2250.041.890738
CTAGTAC1053.8101225E-641.4524463
ACCGTAC700.001053981741.4509741
CACCGTT700.001056559841.4303937
AGTCTAT4300.040.4869961
GACATGG17000.040.1113364
CCGACTA6050.038.36924
ATGCCGG1901.2914825E-1038.156868145
Produced by FastQC (version 0.11.5)