FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2232_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2232_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641252
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC18780.11442484152342236No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC16800.10236088059603278No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTCG554.063024E-665.90838145
GTTACGT1953.6379788E-1240.903231
AGCGAAA3850.039.5510521
GGTTATA4300.035.411991
TTACGTG2302.7284841E-1134.6809352
CTCCGAC2302.7284841E-1134.67354145
CCACGAC3000.031.416328145
TCTCCGC6250.031.319664145
CGAAAGC5400.028.2001553
GCGAAAG5500.027.6874282
GTAGAAA9200.026.7972741
CCGAGGT6300.025.322594
ACGTGTT3201.2387318E-924.9269224
TCTCGTT3501.2914825E-1024.856876145
GCTACTA3352.1118467E-923.8057169
AGACGAC2751.9663639E-723.732083
CGAGGTG7050.023.6572745
GTATATC3702.6011548E-1023.5168441
CGTTTAT1902.1918977E-422.897981
GACGACT2552.7040805E-622.7497064
Produced by FastQC (version 0.11.5)