FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2232_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2232_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233794
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT20580.16680256185392375No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC16100.1304918000898043No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG15100.12238671933888477No Hit
ATTTCATTTATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACTTT14930.12100885561122846No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT14130.11452479101049283No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG13320.10795967560224802No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA13070.10593340541451815No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC902.0503649E-856.38813145
AGTCGAA1151.4094985E-744.1441542
GTTACGT2500.043.5135271
TTACGTG2500.043.5135272
AGCGAAA2800.041.4414521
GTTGACA10200.040.52731
ACATGGT10050.040.410575
CAAGCGG750.001481256638.6786843
AAGTCGA1354.960548E-737.6042821
ACGTGTT2900.037.5116584
CCGACTA4350.036.6780664
GTCGAAG1406.595219E-736.2612723
GCTACTA2501.8189894E-1234.7995349
TATAGTA3000.033.843852
GACGACT1501.1314241E-633.843854
GACTTAA5600.033.671181
GCGAAAG3450.033.633642
GTTATGT7150.033.471941
CCGAGGT3850.032.022944
CCACCGA5250.031.771781
Produced by FastQC (version 0.11.5)