FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2232_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2232_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1756428
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG18660.10623834281849298No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT2550.054.0299261
TCGCGTT953.1413947E-853.420612145
ACCGTAC701.6945984E-551.795611
CGGACCG450.00963306848.3425675
AGTCGAA3250.046.8551032
GACGACT1751.8189894E-1245.5801354
AAGTCGA3400.044.7879681
GTCGAAG3500.043.5083123
AGCGAAA3850.039.5530131
TTACGTG3500.039.3646622
TCTCGTT4200.037.975876145
ACGTGTT3800.036.2569244
CTCCGAC2002.237357E-1036.249702145
CGAAGCG800.002037116336.2497029
TATACTG6950.034.4310385
ATGCCGG3950.033.0377145
GTTGACA15100.030.7343481
ATCTATG8500.030.711751
GCGAAAG5250.030.3867571
GTTATGT9400.030.0855351
Produced by FastQC (version 0.11.5)